| Author's Name: |
Mrs. Ashwini A. Waoo, Swati Khare and Sujata Ganguly |
| Subject Area: |
Life Sciences |
| Subject |
Biotechnology |
| Section |
Research Paper |
Keyword:
Auxin, Callus, Cytokinin, Datura inoxia, Growth hormones, Micro propagation.
Abstract
This paper gives a standardize protocol for micro propagation of Datura inoxia a best plant proven for phytoremediation. Thus focus was given for callus culture. For callus culture of Datura inoxia stem explants were cultured on M. S. basal medium (1962) containing various concentrations and different combinations of growth hormones like KN, NAA and BAP (from 0.1mg/l to 0.5 mg/l. The overall morphological responses of the stem explants indicated that the interaction of these factors leads to shoot-bud proliferation or callus formation. The better response of callus formation was obtained from the stem explants using 0.25-0.5mg/l concentrations of growth hormones. The present study was carried out to analyze the effects of different growth regulators on formation of callus of Datura inoxia in different explants. For this Datura inoxia was cultured in M.S. medium supplemented with various concentrations of KN, NAA and BAP. Culture was incubated on (25 ± 2) oC temperatures and photoperiod of 16 hours while callus was observed on different concentration of Auxin or Cytokinin individually or in combinations. Among experimental conditions, the suitable medium for callus induction from stem explant was NAA (0.5mg/L) in alone and BAP (0.25mg/L) + Kinetin (O.25mg/L) in combinations. There are only limited research works related to tissue culture of Datura inoxia. Taken into account, the phytoremediation capacity of this plant, there is needed to provide efficient tissue culture protocols or micro propagation method for it.